Rat Dynorphin A (Dynorphin A) ELISA Kit

Cat.#:

JL50378

Regulatory Status:

RUO

Alternative:

Pdyn; Dyn-A17

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

4.68-300pg/mL

Sensitivity:

2.39pg/mL

Specificity:

This kit recognizes Rat Dynorphin A in samples. No significant cross-reactivity or interference between Rat Dynorphin A and analogues was observed.

Sample Type:

Serum;plasma;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Dynorphin A

Dynorphin A is a dynorphin, an endogenous opioid peptide that activates the κ-opioid receptor.Its amino acid sequence is Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys, a tridecapeptide.Dynorphin A1–8 is a truncated form of dynorphin A with the amino acid sequence: Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile.Dynorphin A1–8 is an agonist at the mu-, kappa-, and delta-opioid receptors;it has the highest binding affinity for the kappa-opioid receptor.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat Dynorphin A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-104	101
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Rat Dynorphin A and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
1 : 4	Average (%)	93	98	110